The technique of producing the reference values of low-frequency piezothromboelastography in Wistar strain male rats
Abstract
The reference values of low-frequency piezothromboelastography (LFPTEG) in Wistar strain male rats (n=31) weighing 230-330 g, anesthetized with tiletamine-zolazepam (20-40 mg/kg i.m.) + xylazine (5-10 mg/kg i.m.) when taking blood from the left carotid artery in volume of 0,45 ml have been determined. At once after blood sampling the whole blood without citrate was placed in LFPTEG ARP-01M “Mednord” for 8 sec. In the control settings the amplitude delta for adopting a maximum of 1 point, the waiting time of increasing the amplitude for 20 min and calculating density of the clot (DC) according to the formula: DC =An-A0 where n is the desired time point were used. The LFPTEG reference values have been established, and the density of a blood clot at the points t3 (clotting time), t3+5, t3+10, t3+15 min to characterize the dynamics of thrombosis has been calculated. Coagulopathy in rats can be determined by LFPTEG.
References
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REFERENCES
1. Kinzerskiy A.A., Dolgikh V.T., Korzhuk M.S. Temporal and structural dynamics of the low frequency of thrombotic pleasurability male rats Wistar received under xylazine+tiletamine-zolazepam anesthesia, blood sampling from the carotid artery. The certificate of state registration database No. 2016620712 // Bull. No. 6. Published 20.06.2016. (in Russian)
2. Kinzerskiy A.A., Dolgikh V.T., Korzhuk M.S. Normal values of the low frequency pleasurability male rats Wister received under xylazine+tiletamine-zolazepam anesthesia, blood sampling from the carotid artery. The certificate of state registration database No. 2016620346 // Bull. No. 4. Published 20.04.2016. (in Russian)
3. Solovyov M.A., Tyutrin I.I., Udut V.V., Klimenkova V.F. Experience in diagnostics and monitoring of critical violations // Medico-biologichekie i sotsialno-psikhologicheskie problemi bezopasnosti v chrezvachaynikh situatsiyakh. – 2013. – № 4. – P 55-60. (in Russian)
4. Balvers K., Wirtz M.R., van Dieren S., et al. Risk factors for trauma-induced coagulopathy- and transfusion-associated multiple organ failure in severely injured trauma patients // Front. Med. (Lausanne). – 2015. – Vol. 2. № 24. – P. 1-11.
5. Cohen M.J., Call M., Nelson M., et al. Critical role of activated protein C in early coagulopathy and later organ failure, infection and death in trauma patients // Ann. Surg. – 2012. – Vol. 255. № 2. – P. 379-385.
6. Evans J.A., van Wessem K.J., McDougall D., et al. Epidemiology of traumatic deaths: comprehensive population-based assessment // World J. Surg. – 2010. – Vol. 34. №1. – P. 158-163.
7. Gonzalez E., Moore E.E., Moore H.B., et al. Trauma-induced coagulopathy an instition`s 35 years perspective on practice and research // Scand. J. Surg. – 2014. – Vol. 103. № 2. – P. 89-103.
8. Hunt H., Stanworth S., Curry N., et al. Thromboelastography (TEG) and rotational thromboelastometry (ROTEM) for trauma induced coagulopathy in adult trauma patients with bleeding // Cochrane Database Syst. Rev. – 2015. - №2. – P. 322-330.
9. Letson H.L., Dobson G.P. Differential contributions of platelets and fibrinogen to early coagulopathy in a rat model of hemorrhagic shock // Thromb. Res. – 2016. – Vol. 141. – P. 58-65.
10. Maegele M. Acute traumatic coagulopathy: Incidence, risk stratification and therapeutic options // World J. Emerg. Med. – 2010. – Vol. 1. № 1. – P. 12-21.
11. Soreide K., Krüger A.J., Vardal A.L., et al. Epidemiology and contemporary patterns of trauma deaths: changing place, similar pace, older face // World J. Surg. – 2007. – Vol. 31. № 11. – Р. 2092-2103.
12. Teixeira P.G., Inaba K., Hadjizacharia P., et al. Preventable or potentially preventable mortality at a mature trauma center // J. Trauma. – 2007. – Vol. 63. №6. – P. 1338-1346.
13. Winstedt D., Thomas O.D., Nilsson F., et al. Correction of hypothermic and dilutional coagulopathy with concentrates of fibrinogen and factor XIII: an in vitro study with ROTEM // Scand. J. Trauma Resusc. Emerg. Med. – 2014. – Vol. 22. №73. – P. 1-8.
14. Wohlauer M.V., Moore E.E., Harr J., et al. A standardized technique for performing thrombelastography in rodents // Shock. – 2011. – Vol. 36. №55. – P. 524–526.
15. World Health Organization. World health statistics 2013. – URL: http://apps.who.int/iris/bitstream/10665/81965/7/9789244564585_rus.pdf (accessed 08.06.2016)
2. Кинзерский А.А., Долгих В.В., Коржук М.С. Нормальные значения низкочастотной пьезотромбоэластографии крыс-самцов Wistar, полученные под ксилазин+тилетамин-золазепам анестезией при заборе крови из сонной артерии. Свидетельство о государственной регистрации базы данных №2016620346 // Бюл. № 4. Опубликовано 20.04.2016.
3. Соловьев М.А, Тютрин И.И., Удут В.В., Клименкова В.Ф. Опыт диагностики и мониторинга критических нарушений // Медико-биологические и социально-психологические проблемы безопасности в чрезвычайных ситуациях. – 2013. - №4. – С. 55-60.
4. Balvers K., Wirtz M.R., van Dieren S., et al. Risk factors for trauma-induced coagulopathy- and transfusion-associated multiple organ failure in severely injured trauma patients // Front. Med. (Lausanne). – 2015. – Vol. 2. № 24. – P. 1-11.
5. Cohen M.J., Call M., Nelson M. et al. Critical role of activated protein C in early coagulopathy and later organ failure, infection and death in trauma patients // Ann. Surg. – 2012. – Vol. 255, № 2. – P. 379-385.
6. Evans J.A., van Wessem K.J., McDougall D., et al. Epidemiology of traumatic deaths: comprehensive population-based assessment // World J. Surg. – 2010. – Vol. 34, №1. – P. 158-163.
7. Gonzalez E., Moore E.E., Moore H.B., et al. Trauma-induced coagulopathy an instition`s 35 years perspective on practice and research // Scand. J. Surg. – 2014. – Vol. 103. № 2. – P. 89-103.
8. Hunt H., Stanworth S., Curry N., et al. Thromboelastography (TEG) and rotational thromboelastometry (ROTEM) for trauma induced coagulopathy in adult trauma patients with bleeding // Cochrane Database Syst. Rev. – 2015. - №2. – P. 322-330.
9. Letson H.L., Dobson G.P. Differential contributions of platelets and fibrinogen to early coagulopathy in a rat model of hemorrhagic shock // Thromb. Res. – 2016. – Vol. 141. – P. 58-65.
10. Maegele M. Acute traumatic coagulopathy: Incidence, risk stratification and therapeutic options // World J. Emerg. Med. – 2010. – Vol. 1. № 1. – P. 12-21.
11. Soreide K., Krüger A.J., Vardal A.L., et al. Epidemiology and contemporary patterns of trauma deaths: changing place, similar pace, older face // World J. Surg. – 2007. – Vol. 31. № 11. – Р. 2092-2103.
12. Teixeira P.G., Inaba K., Hadjizacharia P., et al. Preventable or potentially preventable mortality at a mature trauma center // J. Trauma. – 2007. – Vol. 63. № 6. – P. 1338-1346.
13. Winstedt D., Thomas O.D., Nilsson F., et al. Correction of hypothermic and dilutional coagulopathy with concentrates of fibrinogen and factor XIII: an in vitro study with ROTEM // Scand. J. Trauma Resusc. Emerg. Med. – 2014. – Vol. 22. №73. – P. 1-8.
14. Wohlauer M.V., Moore E.E., Harr J., et al. A standardized technique for performing thrombelastography in rodents // Shock. – 2011. – Vol. 36. №55. – P. 524–526.
15. World Health Organization. World health statistics 2013. – URL: http://apps.who.int/iris/bitstream/10665/81965/7/9789244564585_rus.pdf (accessed 08.06.2016)
REFERENCES
1. Kinzerskiy A.A., Dolgikh V.T., Korzhuk M.S. Temporal and structural dynamics of the low frequency of thrombotic pleasurability male rats Wistar received under xylazine+tiletamine-zolazepam anesthesia, blood sampling from the carotid artery. The certificate of state registration database No. 2016620712 // Bull. No. 6. Published 20.06.2016. (in Russian)
2. Kinzerskiy A.A., Dolgikh V.T., Korzhuk M.S. Normal values of the low frequency pleasurability male rats Wister received under xylazine+tiletamine-zolazepam anesthesia, blood sampling from the carotid artery. The certificate of state registration database No. 2016620346 // Bull. No. 4. Published 20.04.2016. (in Russian)
3. Solovyov M.A., Tyutrin I.I., Udut V.V., Klimenkova V.F. Experience in diagnostics and monitoring of critical violations // Medico-biologichekie i sotsialno-psikhologicheskie problemi bezopasnosti v chrezvachaynikh situatsiyakh. – 2013. – № 4. – P 55-60. (in Russian)
4. Balvers K., Wirtz M.R., van Dieren S., et al. Risk factors for trauma-induced coagulopathy- and transfusion-associated multiple organ failure in severely injured trauma patients // Front. Med. (Lausanne). – 2015. – Vol. 2. № 24. – P. 1-11.
5. Cohen M.J., Call M., Nelson M., et al. Critical role of activated protein C in early coagulopathy and later organ failure, infection and death in trauma patients // Ann. Surg. – 2012. – Vol. 255. № 2. – P. 379-385.
6. Evans J.A., van Wessem K.J., McDougall D., et al. Epidemiology of traumatic deaths: comprehensive population-based assessment // World J. Surg. – 2010. – Vol. 34. №1. – P. 158-163.
7. Gonzalez E., Moore E.E., Moore H.B., et al. Trauma-induced coagulopathy an instition`s 35 years perspective on practice and research // Scand. J. Surg. – 2014. – Vol. 103. № 2. – P. 89-103.
8. Hunt H., Stanworth S., Curry N., et al. Thromboelastography (TEG) and rotational thromboelastometry (ROTEM) for trauma induced coagulopathy in adult trauma patients with bleeding // Cochrane Database Syst. Rev. – 2015. - №2. – P. 322-330.
9. Letson H.L., Dobson G.P. Differential contributions of platelets and fibrinogen to early coagulopathy in a rat model of hemorrhagic shock // Thromb. Res. – 2016. – Vol. 141. – P. 58-65.
10. Maegele M. Acute traumatic coagulopathy: Incidence, risk stratification and therapeutic options // World J. Emerg. Med. – 2010. – Vol. 1. № 1. – P. 12-21.
11. Soreide K., Krüger A.J., Vardal A.L., et al. Epidemiology and contemporary patterns of trauma deaths: changing place, similar pace, older face // World J. Surg. – 2007. – Vol. 31. № 11. – Р. 2092-2103.
12. Teixeira P.G., Inaba K., Hadjizacharia P., et al. Preventable or potentially preventable mortality at a mature trauma center // J. Trauma. – 2007. – Vol. 63. №6. – P. 1338-1346.
13. Winstedt D., Thomas O.D., Nilsson F., et al. Correction of hypothermic and dilutional coagulopathy with concentrates of fibrinogen and factor XIII: an in vitro study with ROTEM // Scand. J. Trauma Resusc. Emerg. Med. – 2014. – Vol. 22. №73. – P. 1-8.
14. Wohlauer M.V., Moore E.E., Harr J., et al. A standardized technique for performing thrombelastography in rodents // Shock. – 2011. – Vol. 36. №55. – P. 524–526.
15. World Health Organization. World health statistics 2013. – URL: http://apps.who.int/iris/bitstream/10665/81965/7/9789244564585_rus.pdf (accessed 08.06.2016)
Published
2018-04-10
How to Cite
КИНЗЕРСКИЙ, Александр Анатольевич; ДОЛГИХ, Владимир Теретьевич; КОРЖУК, Михаил Сергеевич.
The technique of producing the reference values of low-frequency piezothromboelastography in Wistar strain male rats.
Sibirskij Medicinskij Zurnal (Irkutsk) = Siberian Medical Journal (Irkutsk) 16+, [S.l.], v. 142, n. 3, p. 25-28, apr. 2018.
ISSN 1815-7572. Available at: <http://smj.ismu.baikal.ru/index.php/osn/article/view/148>. Date accessed: 26 july 2026.
Issue
Section
Original research
