THE STATE OF ENDOMETRIAL MICROBIOCENOSIS IN WOMEN OF REPRODUCTIVE AGE WITH CHRONIC ENDOMETRITIS
Abstract
The goal is to determine the microbiological features of chronic endometritis in patients of reproductive age. 76 women with histologically confirmed chronic endometritis (CE) were examined. 47.4% of patients with low activity were included into 1 group, 52.6% of patients with moderate activity of CE-2 group. In order to determine the types and quantities of endometrial microbiota, a polymerase chain reaction (PCR) was used in real time, using a set of reagents "Femoflor-16". In the endometrial microbiota without CE, representatives of normocytosis - Lactobacillus spp. prevailed (85.0%), as well as Eubacterium spp. (60.0%). With respect to the low activity of CE with moderate CE, the endometrial microbiota was characterized by an increase in the frequency of detection of streptococci and staphylococci, 1.2 times, respectively, compared with group I, and 1.3 times as much as enterobacteria. Comparing the frequency of association of microorganisms between groups 1 and 2, a statistically significant difference was found when identifying associations of Mobiluncus spp. + Corynebacterium spp. and Gardnerella vaginalis / Prevotella bivia / Porphyromonas spp. – in moderate CE they were revealed 1.7 times (p <0.05) more seldom.
References
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11. Casari E., Ferrario A., Morenghi E., et al. Gardnerella, Trichomonas vaginalis, Candida, Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the genital discharge of symptomatic fertile and asymptomatic infertile women. // New Microbiol. 2010. Vol. 33(1). P. 69-76.
12. Chen C., Song X., Wei W., et al. The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. // Nat Commun. 2017. Vol. 8(1). P. 875. DOI: 10.1038/s41467-017-00901-0.
13. Cicinelli E., Matteo M., Tinelli R., et al. Chronic endometritis due to common bacteria is prevalent in women with recurrent miscarriage as confirmed by improved pregnancy outcome after antibiotic treatment. // Reprod Sci. 2014. Vol. 21(5). P. 640–647.
14. Haggerty C.L. Evidence for a role of Mycoplasma genitalium in pelvic inflammatory disease. // Curr Opin Infect Dis. 2008. Vol. 21(1). P. 65–69.
15. Khan K.N., Fujishita A., Masumoto H., et al. Molecular detection of intrauterine microbial colonization in women with endometriosis. // Europ J Obstet Gynecol Reprod Bio. 2016. Vol. 199. P. 69-75.
16. Mitchell C.M., Haick A., Nkwopara E., et al. Colonization of the upper genital tract by vaginal bacterial species in nonpregnant women. // Am J Obstet Gynecol. 2015. Vol. 212. P. 611.
REFERENCES
1. Bochkov V., Plekhanov V., Tsydenova T.S. Chronic nonspecific endometritis: epidemiology, etiology, pathogenesis, risk factors, diagnosis. // Bulletin of Buryat state University. Medicina i farmacija. 2015. No 2. P. 30-38. (in Russian)
2. Kupert M.A, Kravchuk L.A., Solodun P.V., Kupert A.F. Microflora of uterus in endometritis after delivery and of vaginae in thevaginitis in pregnant women // Acta Biomedica Scientifica. 2004. № 2-1. P. 169-172. (in Russian)
3. Kupert M.A., Kupert A.F. The new data on pathogenesis of endometritis after delivery // Sibirskij Medicinskij Zurnal (Irkutsk). 2004. Vol. 48. No 7. P. 35-36. (in Russian)
4. Luneva I.S. Chronic endometritis and habitual miscarriage. // Materialy XI Vserossijskogo nauchnogo foruma «Mat' i ditja». Moscow, 2010. P. 128. (in Russian)
5. Lysikov J.A., Rublevsky I.E. Chronic endometritis in women of reproductive age: clinical and microbiological features. // Ohrana materinstva i detstva. 2017. No 2. P. 5-7. (in Russian)
6. Makarov O.V., Kovalchuk L.V., Gankovskaya L.V., et al. Miscarriage, infection, innate immunity. Moscow: GEOTAR-Media, 2007. P. 176. (in Russian)
7. Maltseva L.I., Smolina G.R., Sharipova R.I., et al. Chronic endometritis in the practice of obstetrician-gynecologist. // Rossijskij vestnik akushera-ginekologa. 2015. No 5. P. 102-105. DOI: 10.17116/rosakush2015154102-105 (in Russian)
8. Muravyova V.V., Priputnevich T.V., Yakushevskaya O.V., et al. The role of opportunistic microorganisms in the etiology of reproductive-Age women. // Klinicheskaya mikrobiologia i antimikrobnaya himioterapia. 2015. Vol. 17 (4). P. 318-327. (in Russian)
9. Radzinsky V.E., Petrov A.Yu., Kalinina E.A., et al. Pathogenic characteristics of selected types of chronic endometritis. Kazanskij medicinskij zhurnal. 2017; 98 (1): 27-34. DOI: 10.17750/KMJ2017-27. (in Russian)
10. Yakovenko L.A. Etiological aspects of chronic endometritis (literature review). // International Journal of Applied and Fundamental Research. 2016. No4. P. 574-577. (in Russian)
17. Casari E., Ferrario A., Morenghi E., et al. Gardnerella, Trichomonas vaginalis, Candida, Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the genital discharge of symptomatic fertile and asymptomatic infertile women. // New Microbiol. 2010. Vol. 33(1). P. 69-76.
18. Chen C., Song X., Wei W., et al. The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. // Nat Commun. 2017. Vol. 8(1). P. 875. DOI: 10.1038/s41467-017-00901-0.
19. Cicinelli E., Matteo M., Tinelli R., et al. Chronic endometritis due to common bacteria is prevalent in women with recurrent miscarriage as confirmed by improved pregnancy outcome after antibiotic treatment. // Reprod Sci. 2014. Vol. 21(5). P. 640–647.
20. Haggerty C.L. Evidence for a role of Mycoplasma genitalium in pelvic inflammatory disease. // Curr Opin Infect Dis. 2008. Vol. 21(1). P. 65–69.
21. Khan K.N., Fujishita A., Masumoto H., et al. Molecular detection of intrauterine microbial colonization in women with endometriosis. // Europ J Obstet Gynecol Reprod Bio. 2016. Vol. 199. P. 69-75.
22. Mitchell C.M., Haick A., Nkwopara E., et al. Colonization of the upper genital tract by vaginal bacterial species in nonpregnant women. // Am J Obstet Gynecol. 2015. Vol. 212. P. 611.
2. Куперт M.A., Кравчук Л.А., Солодун П.В., Куперт А.Ф. Микрофлора матки при послеродовом эндометрите и влагалища при вагинитах у беременных женщин // Acta Biomedica Scientifica. 2004. № 2-1. С. 169-172.
3. Куперт М.А., Куперт А.Ф. О патогенезе эндометритов после родов // Сибирский медицинский журнал (Иркутск). 2004. Т. 48. № 7. С. 35-36.
4. Лунева И.С. Хронический эндометрит и привычное невынашивание беременности. // Материалы XI Всероссийского научного форума «Мать и дитя». М., 2010. С. 128.
5. Лызикова Ю.А., Рублевская Е.И. Хронический эндометрит у пациенток репродуктивного возраста: клинико-микробиологические особенности. // Охрана материнства и детства. 2017. №2. С. 5-7.
6. Макаров О.В., Ковальчук Л.В., Ганковская Л.В. и др. Невынашивание беременности, инфекция, врожденный иммунитет. М.: ГЭОТАР-Медиа, 2007. С. 176.
7. Мальцева Л.И., Смолина Г.Р., Шарипова Р.И. и др. Хронический эндометрит в практике акушера-гинеколога. // Российский вестник акушера-гинеколога. 2015. №5. С. 102-105. DOI: 10.17116/rosakush2015154102-105
8. Муравьева В.В., Припутневич Т.В., Якушевская О.В. и др. Роль условно-патогенных микроорганизмов в этиологии хронического эндометрита у женщин репродуктивного возраста. // Клиническая микробиология и антимикробная химиотерапия. 2015. Т. 17. №4. С. 318-327.
9. Радзинский В.Е., Петров Ю.А., Калинина Е.А. и др. Патогенетические особенности макротипов хронического эндометрита. // Казанский медицинский журнал. 2017. Т. 98. №1. С. 27-34. DOI:10.17750/ KMJ2017-27.
10. Яковенко Л.А. Этиологические аспекты хронического эндометрита (обзор литературы). // International Journal of Applied and Fundamental Research. 2016. №4. С. 574-577.
11. Casari E., Ferrario A., Morenghi E., et al. Gardnerella, Trichomonas vaginalis, Candida, Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the genital discharge of symptomatic fertile and asymptomatic infertile women. // New Microbiol. 2010. Vol. 33(1). P. 69-76.
12. Chen C., Song X., Wei W., et al. The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. // Nat Commun. 2017. Vol. 8(1). P. 875. DOI: 10.1038/s41467-017-00901-0.
13. Cicinelli E., Matteo M., Tinelli R., et al. Chronic endometritis due to common bacteria is prevalent in women with recurrent miscarriage as confirmed by improved pregnancy outcome after antibiotic treatment. // Reprod Sci. 2014. Vol. 21(5). P. 640–647.
14. Haggerty C.L. Evidence for a role of Mycoplasma genitalium in pelvic inflammatory disease. // Curr Opin Infect Dis. 2008. Vol. 21(1). P. 65–69.
15. Khan K.N., Fujishita A., Masumoto H., et al. Molecular detection of intrauterine microbial colonization in women with endometriosis. // Europ J Obstet Gynecol Reprod Bio. 2016. Vol. 199. P. 69-75.
16. Mitchell C.M., Haick A., Nkwopara E., et al. Colonization of the upper genital tract by vaginal bacterial species in nonpregnant women. // Am J Obstet Gynecol. 2015. Vol. 212. P. 611.
REFERENCES
1. Bochkov V., Plekhanov V., Tsydenova T.S. Chronic nonspecific endometritis: epidemiology, etiology, pathogenesis, risk factors, diagnosis. // Bulletin of Buryat state University. Medicina i farmacija. 2015. No 2. P. 30-38. (in Russian)
2. Kupert M.A, Kravchuk L.A., Solodun P.V., Kupert A.F. Microflora of uterus in endometritis after delivery and of vaginae in thevaginitis in pregnant women // Acta Biomedica Scientifica. 2004. № 2-1. P. 169-172. (in Russian)
3. Kupert M.A., Kupert A.F. The new data on pathogenesis of endometritis after delivery // Sibirskij Medicinskij Zurnal (Irkutsk). 2004. Vol. 48. No 7. P. 35-36. (in Russian)
4. Luneva I.S. Chronic endometritis and habitual miscarriage. // Materialy XI Vserossijskogo nauchnogo foruma «Mat' i ditja». Moscow, 2010. P. 128. (in Russian)
5. Lysikov J.A., Rublevsky I.E. Chronic endometritis in women of reproductive age: clinical and microbiological features. // Ohrana materinstva i detstva. 2017. No 2. P. 5-7. (in Russian)
6. Makarov O.V., Kovalchuk L.V., Gankovskaya L.V., et al. Miscarriage, infection, innate immunity. Moscow: GEOTAR-Media, 2007. P. 176. (in Russian)
7. Maltseva L.I., Smolina G.R., Sharipova R.I., et al. Chronic endometritis in the practice of obstetrician-gynecologist. // Rossijskij vestnik akushera-ginekologa. 2015. No 5. P. 102-105. DOI: 10.17116/rosakush2015154102-105 (in Russian)
8. Muravyova V.V., Priputnevich T.V., Yakushevskaya O.V., et al. The role of opportunistic microorganisms in the etiology of reproductive-Age women. // Klinicheskaya mikrobiologia i antimikrobnaya himioterapia. 2015. Vol. 17 (4). P. 318-327. (in Russian)
9. Radzinsky V.E., Petrov A.Yu., Kalinina E.A., et al. Pathogenic characteristics of selected types of chronic endometritis. Kazanskij medicinskij zhurnal. 2017; 98 (1): 27-34. DOI: 10.17750/KMJ2017-27. (in Russian)
10. Yakovenko L.A. Etiological aspects of chronic endometritis (literature review). // International Journal of Applied and Fundamental Research. 2016. No4. P. 574-577. (in Russian)
17. Casari E., Ferrario A., Morenghi E., et al. Gardnerella, Trichomonas vaginalis, Candida, Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the genital discharge of symptomatic fertile and asymptomatic infertile women. // New Microbiol. 2010. Vol. 33(1). P. 69-76.
18. Chen C., Song X., Wei W., et al. The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. // Nat Commun. 2017. Vol. 8(1). P. 875. DOI: 10.1038/s41467-017-00901-0.
19. Cicinelli E., Matteo M., Tinelli R., et al. Chronic endometritis due to common bacteria is prevalent in women with recurrent miscarriage as confirmed by improved pregnancy outcome after antibiotic treatment. // Reprod Sci. 2014. Vol. 21(5). P. 640–647.
20. Haggerty C.L. Evidence for a role of Mycoplasma genitalium in pelvic inflammatory disease. // Curr Opin Infect Dis. 2008. Vol. 21(1). P. 65–69.
21. Khan K.N., Fujishita A., Masumoto H., et al. Molecular detection of intrauterine microbial colonization in women with endometriosis. // Europ J Obstet Gynecol Reprod Bio. 2016. Vol. 199. P. 69-75.
22. Mitchell C.M., Haick A., Nkwopara E., et al. Colonization of the upper genital tract by vaginal bacterial species in nonpregnant women. // Am J Obstet Gynecol. 2015. Vol. 212. P. 611.
Published
2019-01-31
How to Cite
АКПЕРБЕКОВА, Самира Алекпер кызы.
THE STATE OF ENDOMETRIAL MICROBIOCENOSIS IN WOMEN OF REPRODUCTIVE AGE WITH CHRONIC ENDOMETRITIS.
Sibirskij Medicinskij Zurnal (Irkutsk) = Siberian Medical Journal (Irkutsk) 16+, [S.l.], v. 154, n. 3, p. 40-43, jan. 2019.
ISSN 1815-7572. Available at: <http://smj.ismu.baikal.ru/index.php/osn/article/view/308>. Date accessed: 01 jan. 2026.
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