ATHEROSCLEROTIC PLAQUES FORMATION ON THE SURFAGE OF AORTA (EXPERIMENTAL STUDY)

  • Николай Петрович Судаков Irkutsk Scientific Center of Surgery and Traumatology; Limnological Institute, Siberian Branch, Russian Academy of Sciences; Irkutsk State University
  • Игорь Викторович Клименков Irkutsk Scientific Center of Surgery and Traumatology; Limnological Institute, Siberian Branch, Russian Academy of Sciences; Irkutsk State University
  • Татьяна Павловна Попкова Irkutsk Scientific Center of Surgery and Traumatology
  • Евгения Александровна Лозовская Irkutsk Scientific Center of Surgery and Traumatology
  • Сергей Борисович Никифоров Irkutsk Scientific Center of Surgery and Traumatology
  • Олег Аронович Гольдберг Irkutsk Scientific Center of Surgery and Traumatology
  • Борис Георгиевич Пушкарев Irkutsk Scientific Center of Surgery and Traumatology
  • Ольга Владимировна Чупрова Irkutsk State University
  • Елена Владимировна Вантеева Irkutsk State University
  • Юрий Михайлович Константинов Irkutsk State University; Siberian Institute of Plant Physiology and Biochemistry SB RAS

Abstract

This work is devoted to the study of the in vivo stages of development of lipid infiltration of foam cells and the
formation of atherosclerotic plaques from them in an experiment on “Chinchilla” rabbits. Using laser confocal microscopy
we performed a three-dimensional analysis of rabbit aorta preparations (135 days of atherogenic diet) stained with Nile red.
Experimental evidence suggests that atherosclerotic plaques form in areas of the aorta-attached single monocyte/macrophage
that begins to consume lipids, turning into foam cells. The topography of the distribution of these cells on the inner surface of
the aorta determines the shape of the developing atherosclerotic plaque. Further plaque formation occurs due to an increase
in lipid accumulations of already existing foam cells, the addition of new ones, the formation of lateral contacts between them
and, further, multilayer structures. In general, the data obtained will serve as the basis for the creation of new methods of
diagnosis, prevention and treatment of atherosclerosis.

References

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6. Angheloiu G.O., Haka A.S., Georgakoudi I., et al. Detection of coronary atherosclerotic plaques with superficial proteoglycans and foam cells using real-time intrinsic fluorescence spectroscopy // Atherosclerosis. 2011. Vol. 215. №1. P.96-102. DOI: 10.1016/j.atherosclerosis.2010.11.020.
7. Butcher M.J., Herre M., Ley K., Galkina E. Flow cytometry analysis of immune cells within murine aortas // J. Vis Exp. 2011. Vol. 53. P.2848. DOI: 10.3791/2848.
8. Coia H., Ma N., He A.R., Kallakury B., et al. Detection of a lipid peroxidation-induced DNA adduct across liver disease stages // Hepatobiliary Surg Nutr. 2018. Vol. 7. №2. P.85-97. DOI:10.21037/hbsn.2017.06.01.
9. Koniari I., Mavrilas D., Papadaki H., et al. Structural and biomechanical alterations in rabbit thoracic aortas are associated with the progression of atherosclerosis // Lipids Health Dis. 2011. Vol. 10. P.125. DOI: 10.1186/1476-511X-10-125.
10. Neel B.A., Zong H., Backer J.M., Pessin J.E. Identification of Atypical Peri-Nuclear Multivesicular Bodies in Oxidative and Glycolytic Skeletal Muscle of Aged and Pompe’s Disease Mouse Models // Front Physiol. 2015. Vol. 6. P.393. DOI: 10.3389/
fphys.2015.00393.
11. Suh W.M., Seto A.H., Margey R.J., Cruz-Gonzalez I., Jang I.K. Intravascular detection of the vulnerable plaque // Circ Cardiovasc Imaging. 2011. Vol. 4. №2. P.169-178. DOI: 10.1161/ CIRCIMAGING.110.958777.
12. Sun B., Shi Z., Pu J., et al. Effects of mechanical thrombectomy for acute stroke patients with etiology of large arteryatherosclerosis // J Neurol Sci. 2018. Vol. 396. P.178-183. DOI: 10.1016/j.jns.2018.10.017.
13. Xu S., Huang Y., Xie Y., et al. Evaluation of foam cell formation in cultured macrophages: an improved method with Oil Red O staining and DiI-oxLDL uptake // Cytotechnology.2010. Vol. 62. №5. P.473-481. DOI: 10.1007/s10616-010-9290-0.
Published
2019-02-16
How to Cite
СУДАКОВ, Николай Петрович et al. ATHEROSCLEROTIC PLAQUES FORMATION ON THE SURFAGE OF AORTA (EXPERIMENTAL STUDY). Sibirskij Medicinskij Zurnal (Irkutsk) = Siberian Medical Journal (Irkutsk) 16+, [S.l.], v. 155, n. 4, p. 17-20, feb. 2019. ISSN 1815-7572. Available at: <http://smj.ismu.baikal.ru/index.php/osn/article/view/321>. Date accessed: 26 july 2026.
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Vol 155 No 4 (2018)
Section
Original research
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