THE METHOD OF MATHEMATICAL PROCESSING OF PHOSPHOLIPID ZONES OBTAINED BY THIN LAYER CHROMATOGRAPHY
Abstract
Aim: to develop an algorithm for processing phospholipid chromatograms to quantify the content of lipid componentsin the erythrocyte membrane.Methods. Chromatograms of phospholipids (phosphatidylcholine and phosphatidylethanolamine) of erythrocytemembranes obtained by thin layer chromatography were used as an object of study. Erythrocyte membranes were incubatedin physiological solution and Mexidol solution. Elution of phospholipids was performed in a single-step vertical method.Iodine vapor served as an indicator.Results. The presented program makes it possible to evaluate various quantitative characteristics of phospholipidchromatograms. The meaning of the proposed algorithm is to select and record each of the chromatographic spots in a separatefile in the form of a square matrix and combine them in the Photoshop graphic editor. After reading-in process, scannedsamples of phospholipid spots from prepared files with an extension (.pcx) are automatically processed in the Mathcad-2001Professional mathematical package to determine the area and/or mass of erythrocyte membrane lipid components takinginto account the intensity of dark gray phospholipid spots. The program provides for the export of the results of processingof chromatorphic spots not only in digital, but also in illustrated forms.Conclusion. The proposed algorithms for mathematical processing of chromatograms can be used in various aspects ofcomparative analysis of the content of lipid components of the erythrocyte membrane, both in clinical and experimentalstudies.
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