PROFILE AND ASSOCIATION OF GENE EXPRESSION IN THE SAMPLES OF THE HIP JOINT SYNOVIAL MEMBRANE IN PATIENTS WITH COXARTHROSIS STAGE III–IV
Abstract
In the treatment of severe osteoarthritis, endoprosthesis replacement is widely used. This method makes it is possible to select samples of synovial membrane for the research. In osteoarthritis, all components that form the joint are affected. However, if the role of cartilage tissue has been actively studied for many years, the study of the role of synovial membrane has not been given enough attention. In this regard, the aim of the study was to identify the gene expression profile by transcript content in intraoperative samples of synovial tissue of patients with stage III osteoarthritis of hip joint. During total hip replacement surgery, the fragments of synovial membrane were collected into solution that prevents hydrolysis of nucleic acids. After degradation and homogenization of the obtained biopsies, RNA was isolated using real time PCR with specific primers. The expression profile of a sample of genes involved in connective tissue metabolism in intraoperatively taken 10 samples of synovial membrane of patients with stage III osteoarthritis of hip joint was determined. The following genes are most actively expressed in the samples of synovial membrane in osteoarthritis of hip joint: FGFR1, PDGFA, NAA20, PDGFB, Dio1, ESR1, ESR2. The average expression level was registered for CALCR, Dio2, AANAT, PTH2R, NAT2. Significantly fewer transcripts in the synovial tissue were kept for the FGFR3 gene, Dio3, NAT1, PTH1R, and GDF5. The data of the correlation analysis indicates the close interconnection of metabolic pathways which include selected candidate genes. Identified tension of a network interactions indicates a decline in metabolic “flexibility” processes in the synovial tissue in terms of pathology.
References
2. Булыгин Г.В., Камзалакова Н.И., Андрейчиков А.В. Метаболические основы регуляции иммунного ответа. Новосибирск, 1999. 346 с.
3. Корочина К.В., Чернышева Т.В., Корочина И.Э., Полякова В.С., Аверьянов А.А. Патогистологические особенности синовиальной оболочки пациентов с гонартрозом поздних стадий // Оренбургский медицинский вестник. 2017. Т. V. № 3. С. 15–18.
4. Соколов В.В., Нарциссов Р.П., Иванова Л.А. Цитохимия ферментов в профпатологии. М., 1975. 244 с.
5. Capellino S., Straub R.H., Cutolo M. Aromatase and regulation of the estrogen-to-androgen ratio in synovial tissue inflammation: common pathway in both sexes // Ann. N.-Y. Acad. Sci. 2014. Vol. 1317. P. 24–31. DOI: 10.1111/nyas.12398.
6. Lugo L., Villalvilla A., Gómez R., Bellido M., Sánchez-Pernaute O., Largo R., Herrero-Beaumont G., Roman-Blas J.A. Effects of PTH [1-34] on synoviopathy in an experimental model of osteoarthritis preceded by osteoporosis // Osteoarthritis Cartilage. 2012. Vol. 20 (12). P. 1619–1630. DOI: 10.1016/j.joca.2012.08.010.
7. Sellam J., Berenbaum F. The role of synovitis in pathophysiology and clinical symptoms of osteoarthritis // Nat. Rev. Rheumatol. 2010. Vol. 6. P. 625–635.
REFERENCES
1. Aliyev V.A. Correlation between leukocyte enzymes in normal conditions and under toxicological effect // Laboratornoe delo. 1984. № 8. P. 465–468. (in Russian)
2. Bulygin G.V., Kamzalakova N.I., Andreychikov A.V. Metabolic basics of immune response regulation. Novosibirsk, 1999. 346 p. (in Russian)
3. Korochina K.V., Chernysheva T.V., Korochina I.E., Polyakova V.S., Averyanov A.A. Pathological peculiarities of synovial membrane in patients with gonarthrosis at advanced stages // Orenburgskiy meditsinskiy vestnik. 2017. Vol. 5 (3). P. 15–18. (in Russian)
4. Sokolov V.V., Nartsissov R.P., Ivanova L.A. Cytochemistry of enzymes in occupational pathology. Moscow, 1975. 244 p. (in Russian)
5. Capellino S., Straub R.H., Cutolo M. Aromatase and regulation of the estrogen-to-androgen ratio in synovial tissue inflammation: common pathway in both sexes // Ann. N.-Y. Acad. Sci. 2014. Vol. 1317. P. 24–31. DOI: 10.1111/nyas.12398.
6. Lugo L., Villalvilla A., Gómez R., Bellido M., Sánchez-Pernaute O., Largo R., Herrero-Beaumont G., Roman-Blas J.A. Effects of PTH [1-34] on synoviopathy in an experimental model of osteoarthritis preceded by osteoporosis // Osteoarthritis Cartilage. 2012. Vol. 20 (12). P. 1619–1630. DOI: 10.1016/j.joca.2012.08.010.
7. Sellam J., Berenbaum F. The role of synovitis in pathophysiology and clinical symptoms of osteoarthritis // Nat. Rev. Rheumatol. 2010. Vol. 6. P. 625–635.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
